85-4496-95　Lipoprotein-associated Phospholipase A2 (Lp-PLA2) BioAssay™ ELISA Kit (Human) 96Tests　351661

特徴

• Sample Type:Serum, plasma, tissue homogenates
• Intended Use:For the quantitative determination of human lipoprotein-associated phospholipase A2 (Lp-PLA2) concentrations in serum, plasma, tissue homogenates.
• Sensitivity:0.39ng/ml
• Range:1.56-100ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human Lp-PLA2. No significant cross-reactivity or interference between human Lp-PLA2 and analogues was observed.
• Principle of the Assay:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Lp-PLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lp-PLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-PLA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-PLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:351661A: Microtiter Strips, 12x8 Microwell Strips. Pre-coated; ready to use. 351661B: Standard (lyophilized), 2 vials351661C: Biotin-Antibody (100x Concentrate), 1x120ul351661D: HRP-Avidin, (100x Concentrate), 1x120ul351661E: Biotin-Antibody Diluent, 1x15ml351661F: HRP-Avidin Diluent, 1x15ml351661G: Sample Diluent, 1x50ml351661H: Wash Buffer (25X Concentrate), 1x20ml351661J: TMB Substrate, 1x10ml351661K: Stop Solution, 1x10ml
• Storage and Stability:Store all kit components at 4°C. Unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for 1 month provided this is within the expiration date of the kit
• Opened Kit: Coated plate may be stored at 4°C for up to 1 month, sealed in the aluminum foil bag. Standard, Biotin-Antibody and HRP-Avidin may be stored at 4°C or -20°C for up to 1 month. The rest of the components may be stored at 4°C for up to 1 month
• For maximum recovery of contents, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Summary:1. Prepare reagents, standards and samples as instructed
• 2. Add 100ul standard or sample to each well. Incubate for 2 hours at 37°C3. Remove the liquid of each wee; do not wash
• 4. Add 100ul Biotin-Antibody (1x) to each well. Incubate for 1 hour at 37°C5. Aspirate and wash 3 times
• 6. Add 100ul HRP-Avidin (1x) to each well. Incubate for 1 hour at 37°C7. Aspirate and wash 5 times
• 8. Add 90ul TMB substrate to each well Incubate for 15-30 minutes at 37°C. Protect from light
• 9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes