85-4496-55　Hepatocyte Growth Factor Receptor (c-MET/HGFR) BioAssay™ ELISA Kit (Human) 48Tests　351648

特徴

• The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
• Sample Type:Serum, plasma, tissue homogenates
• Intended Use:For the quantitative determination of human hepatocyte growth factor receptor (c-MET/ HGFR) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:0.39ng/ml
• Range:1.56-100ng/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human c-MET. No significant cross-reactivity or interference between human c-MET and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for c-MET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any c-MET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for c-MET is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of c-MET bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsBiotin-antibody (100X), 1x120ulHRP-avidin (100X), 1x120ulBiotin-antibody Diluent, 1x15mlHRP-avidin Diluent, 1x15mlSample Diluent, 1x50mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.