85-4496-45　［取扱停止］Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) BioAssay™ ELISA Kit (Human) 24Tests　351644

特徴

• This protein is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
• Sample Type:Serum, plasma, cell culture supernates, tissue homogenates
• Intended Use:For the quantitative determination of human granulocyte-macrophage colony stimulating factor (GM-CSF) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
• Sensitivity:3.9pg/ml
• Range:15.6-1000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of human GM-CSF. No significant cross-reactivity or interference between human GM-CSF and analogues was observed.
• Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsBiotin-antibody (100X), 1x120ulHRP-avidin (100X), 1x120ulBiotin-antibody Diluent, 1x15mlHRP-avidin Diluent, 1x15mlSample Diluent, 1x50mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.