85-4496-33　［取扱停止］Folic Acid (FA) BioAssay™ ELISA Kit (Bovine) 24Tests　351640

特徴

• Sample Type:Serum, plasma and tissue homogenates
• Intended Use:For the quantitative determination of endogenic bovine folic acid (FA) concentrations in serum, plasma and tissue homogenates.
• Sensitivity:1.95pg/ml
• Range:7.8-500pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of bovine FA. No significant cross-reactivity or interference between bovine FA and analogues was observed.
• Test Principle:This BioAssay™ employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for FA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated FA. A competitive inhibition reaction is launched between FA (Standards or samples) and biotin-conjugated FA with the pre-coated FA antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of FA bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard, 2x0.5mlBiotin-conjugate (10X), 1x600ulHRP-avidin (100X), 1x120ulBiotin-conjugate Diluent, 1x10mlHRP-avidin Diluent, 1x10mlSample Diluent, 2x20mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.