85-4495-71　Chorionic gonadotropin (AhCGAb) BioAssay™ ELISA Kit (Human) 96Tests　351620

特徴

• Sample Type:Serum
• Intended Use:This immunoassay kit allows for the in vitro qualitative determination of human AhCGAb (IgG) concentrations in serum.
• Specificity:This assay recognizes human AhCGAb(IgG). No significant cross-reactivity or interference was observed.
• Test Principle:The microtiter plate provided in this kit has been pre-coated with specific antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-human IgG and incubate again. Substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm± 2nm. Calculate the valence of human AhCGAb(IgG) in the samples.
• Kit Components:Assay plate, 1Sample Diluent, 1x20mlHRP-conjugate, 1x10mlWash Buffer (25X), 1x20mlPositive Control, 1x0.8mlNegative Control, 1x0.8mlSubstrate A, 1x5mlSubstrate B, 1x5mlStop Solution, 1x5ml
• Sample Volume:50-100ul
• Detection Wavelength:450nm
• Precision:Intra-assay: CV% <15%Inter-assay: CV% <15%
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.