85-4495-22　Apolipoprotein A5 (Apo-A5) BioAssay™ ELISA Kit (Porcine) (Apo-A5, APOAV, RAP3, Regeneration-associated Protein 3) 48Tests　351604

特徴

• Sample Type:Serum, plasma, tissue homogenates
• Intended Use:For the quantitative determination of porcine Apolipoprotein A5 (apo-A5) concentrations in serum, plasma, tissue homogenates.
• Sensitivity:7.8pg/ml
• Range:31.25-3000pg/ml
• Specificity:This assay has high sensitivity and excellent specificity for detection of porcine APO-A5. No significant cross-reactivity or interference between porcine APO-A5 and analogues was observed.
• Test Principle:This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APO-A5. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APO-A5. The competitive inhibition reaction is launched between with pre-coated APO-A5 and APO-A5 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APO-A5 in the samples. The color development is stopped and the intensity of the color is measured.
• Kit Components:Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsHRP-conjugate (100X), 1x60ulHRP-conjugate Diluent, 1x10mlSample Diluent, 2x20mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.