85-4493-95　TWF1, Recombinant, Human, aa1-252, His-Tag (Twinfilin-1, PTK9, Protein Tyrosine Kinase 9) 250ug　351552

特徴

• TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of ∼20 residues. The two ADF-H domains are ∼20% homologous to ADF/cofilin and to each other.
• Source:Recombinant protein corresponding to aa1-252 from human TWF1, fused to His-tag at N- terminal expressed in E. coli.
• Molecular Weight:~31.5kD (277aa)
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMMLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.