85-4492-91　SlyD, Recombinant, E.coli, aa1-196 (FKBP-type peptidyl-prolyl cis-trans isomerase) 500ug　351497

特徴

• SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N- terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
• Source:Recombinant protein corresponding to aa1-196 from E. coli SlyD expressed in E. coli.
• Molecular Weight:~21kD (196aa)
• Specific Activity:~220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA/minute at 25°C in Tris-HCL, pH 8.0, using chymotrypsin.
• Activity Assay:1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200mM Tris-HCL, pH 8.0, and 20nM chymotrypsin
• 2. Add 10ul of recombinant SlyD protein with 1ug in assay buffer
• 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer
• 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
• AA Sequence:MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.