CSF1, also known as macrophage colony-stimulating factor 1, is a hematopoietic growth factor that is the primary regulator of the survival, proliferation, differentiation and function of mononuclear phagocytes. It is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide linked homodimer. This protein plays important roles in innate immunity, cancer and inflammatory diseases, including systemic lupus erythematosus, arthritis, atherosclerosis and obesity. In several conditions, activation of macrophages involves a CSF1 autocrine loop. In addition, secreted and cell-surface isoforms of CSF1 can have differential effects in inflammation and immunity.
Recombinant protein corresponding to aa33-255 from human CSF1, fused to His-tag at C-terminal expressed in insect cell.
Biological Activity:Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range <3ng/ml.
Endotoxin:<1EU/1ug (LAL)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, human from insect cell line
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
