85-4489-82　Interleukin-3, Recombinant, Human, aa20-152, His-Tag (IL-3, Multipotential colony-stimulating factor, Multi-CSF, Mast cell growth factor, MCGF) 500ug　351326

特徴

• Interleukin-3(IL-3) stimulates the proliferation of hematopoietic pluripotent progenitor cells. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 exerts its biological activities through binding to specific cell surface receptors. IL-3 receptor is a complex consisting of alpha and beta subunits, which in turn induces activation of JAK2/ STAT5, induction of c-myc, and activation of the Ras pathway. Human IL3 is a protein of 15-17kD (133aa). It is synthesized as a precursor containing a hydrophobic secretory signal sequence of 19aa. IL3 contains two putative glycosylation sites at positions 15 and 70 and contains a single disulfide bond (Cys16/84).
• Source:Recombinant protein corresponding to aa20-152 from human Interleukin-3 fused to His-tag at N-terminal, expressed in E. coli.
• Molecular Weight:~17.3kD (154aa)
• Biological Activity:Measured in a cell proliferation assay using TF1 human erythroleukemic cells, the ED50 for this effect is <0.53ng/ml.
• Activity Assay:1. Cell line: TF1 (human erythroleukemic cell)2. Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF3. Assay medium: 10% FBS RPMI 1640 without Gm-CSF4. Cell density: 2x10e4cells/well (96 well plate, final volume 100ul) 5. Starvation: 24hr, Assay medium6. Incubation time: 24hr (after sample treatment)7. Concentration range: 0.048-25ng/ml8. Detection method: MTT assay
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
• Endotoxin: ~1EU/1ug of protein (LAL)
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.