85-4489-77　Interleukin-15, Recombinant, Human, aa49-162, His-Tag (IL-15) 100ug　351323

特徴

• Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
• Source:Recombinant protein corresponding to aa49-162 from human Interleukin-15 fused to His-tag at N-terminal, expressed in E. coli.
• Molecular Weight:~13.9kD (123aa)
• Biological Activity:Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells, the ED50 for this effect is <2.5ng/ml.
• Activity Assay:1. Cell line: CTLL2 (mouse cytotoxic Tcell)2. Maintenance Condition: 10% FBS RPMI 1640 with hIL23. Assay Medium: 10% FBS RPMI 1640 without hIL24. Cell Density: 2x10e4 cells/well (96 well plate, final volume 100ul) 5. Starvation: 24hr, Assay medium6. Incubation Time: 24hr (after sample treatment)7. Concentration Range: 0.039-40ng/ml8. Detection Method: MTT assay
• AA Sequence:MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH
• Endotoxin: ~1EU/1ug of protein (LAL)
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.