85-4489-25　Idnk, Recombinant, E. coli, aa1-187, His-Tag (D-gluconate Kinase, thermosensitive, GntV) 100ug　351296

特徴

• D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187aa protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype.
• Source:Recombinant protein corresponding to aa1-187 from E. coli idnk, fused to His-tag at N-terminal expressed in E. coli.
• Molecular Weight:~23.4kD (210aa)
• Specific Activity:>80units/mg, in which one unit will convert 1umole of D-gluconate to 6-phospho-D-gluconate/minute at pH 8.0 at 37°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.