85-4487-37　DHFR, Recombinant, Mouse, aa1-187, His-Tag (Dihydrofolate reductase) 100ug　351189

特徴

• Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)- resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
• Source:Recombinant protein corresponding to aa1-187 from mouse DHFR, fused to His-tag at N-terminal expressed in E. coli.
• Molecular Weight:~23.6kD (207aa)
• Specific Activity:>2000pmol/min/ug, and is defined as the amount of enzyme that converts 1pmole of dihydrofolic acid to tetrahydrofolic acid/minute at pH 6.5 at 25°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.