85-4486-13　Carbonic anhydrase 1, Recombinant, Human, aa1-261, His-Tag (CA1, CA-I, CAB) 100ug　351120

特徴

• Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ↔ HCO3− + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life.
• Source:Recombinant protein corresponding to aa1-261 from human carbonic anhydrase, fused to His-tag expressed in E. coli.
• Molecular Weight:~31kD (281aa)
• Specific Activity:>300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of 4-nitrophenyl acetate to 4-nitrophenol/minute at pH 8.0 at 37°C.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.