85-4485-89　BMP-2, Active, Recombinant, Human, aa283-396 (Bone morphogenetic protein 2, BMP-2A) 250ug　351107

特徴

• BMP-2 (Bone morphogenetic protein 2) is multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.
• Source:Recombinant protein corresponding to aa283-396 from human BMP-2, expressed in E. coli.
• Molecular Weight:~13kD (115aa)
• Biological Activity:Measured by its ability to induce alkaline phosphatase production using ATDC5 mouse chondrogenic cells, the ED50 for this effect is <57ng/ml.
• Activity Assay:1. Cell line: ATDC5 (mouse chondrogenic cell)2. Maintenance Condition: 10% FBS RPMI 16403. Assay medium: 1% FBS RPMI 16404. Cell Density: 2x10e4 cells/well (96 well plate, final volume 100ul) 5. Starvation: 24hr, Assay medium6. Incubation Time: 48hr (after sample treatment)7. Concentration Range: 1ng/ml-10ug/ml8. Detection method: ALP assay
• AA Sequence:MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
• Endotoxin: ~1EU/1ug (LAL)
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.