Recombinant protein corresponding to aa570-756 of human LOXL4, fused to His-tag at C-terminal; expressed in E. coli.
Molecular Weight:~23kD
Assay Conditions:Enzymatic activity was confirmed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL4 (0.25-1ng/ul) was incubated with 3mM 1,5 diaminopentane, 0.4mM AmplexRed and 0.2unit/ul peroxidase in 100ul of the assay buffer (1.2M urea, 50mM borate, pH 8.8) for 15 min at 30°C. Fluorescence intensity is measured every 7 min at 530ex/590em.
Applications:Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified (~90%)
Form：Supplied as a liquid in 9mM Na2HPO4/KH2 PO4, pH 7.4, 124mM sodium chloride, 2.4mM potassium chloride, 10% glycerol.
