85-4457-71　［取扱停止］Tumor Necrosis Factor, active (Tnf), Recombinant, Mouse 100ug　348268

特徴

• Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000250}.
• Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1ng/ml, corresponding to a specific activity of ＞ 1.0x10e7 IU/mg in the presence of actinomycin D.
• Sequence:MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
• Molecular Weight:17.4kD
• PubMed ID:2836146; 3898078; 2419912; 2989794; 3040015; 3684584; 7560085; 9089109; 14656967; 2777790; 2268312; 3349526; 10089307
• Gene Name:Tnf,Tnfa,Tnfsf2
• Swiss Prot:P06804
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.