85-4457-63　Tumor Necrosis Factor, active (TNF), Recombinant, Human 10ug　348265

特徴

• Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line.
• Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of ＞ 2.0x10e7 IU/mg in the presence of actinomycin D.
• Sequence:MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
• Molecular Weight:18.3kD
• PubMed ID:3555974; 6392892; 3883195; 2995927; 3856324; 3932069; 8499947; 10202016; 14656967; 15489334; 8631363; 8597870; 10205166; 2009860; 1402651; 9034191; 10e369255; 16829952; 16829951; 22517918; 23396208; 2922050; 1964681; 2551905; 9488135; 9442056; 12746914; 12915457
• Gene Name:TNF, TNFA, TNFSF2
• Swiss Prot:P01375
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.