85-4457-52　［取扱停止］Tumor Necrosis Factor Receptor Superfamily Member 1B, active (TNFRSF1B), Recombinant, Human 20ug　348262

特徴

• Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. {ECO:0000269|PubMed:12370298}.
• Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2ug/ml, corresponding to a specific activity of ＞ 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.
• Sequence:MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
• Molecular Weight:20.0kD
• PubMed ID:2172983; 2160731; 8661109; 14688072; 16710414; 15489334; 2173696; 8015639; 2153136; 1966549; 2166946; 11197692; 1328224; 8069916; 12370298; 25456591; 10206649; 11762942; 12161545
• Gene Name:TNFRSF1B,TNFBR,TNFR2
• Swiss Prot:P20333
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.