85-4455-76　［取扱停止］Pro-Epidermal Growth Factor, active (EGF), Recombinant, Human 500ug　348204

特徴

• EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). {ECO:0000269|PubMed:10964941, ECO:0000269|PubMed:17671655}.
• Biological Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of ＞1.0x10e7 IU/mg.
• Sequence:NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
• Molecular Weight:6.2kD
• PubMed ID:3491360; 14702039; 15815621; 15489334; 300079; 2789514; 22171320; 1522591; 10964941; 17671655; 21439629; 11438527; 12297050; 12620237; 21029725; 20837704
• Gene Name:EGF
• Swiss Prot:P01133
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.