The different signal transduction pathways involved in the up-regulation of cAMP by the peptide hormone relaxin (RLX). When relaxin binds to its G protein-coupled receptor, LGR7 or LGR8, a G protein signaling pathway is activated leading to stimulation of adenylate cyclase (AC) and an increase in cAMP. Binding of relaxin to its receptor also may activate a tyrosine kinase pathway that inhibits the activity of a phosphodiesterase (PDE) that degrades cAMP .
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
