Purified, His-tagged Staphylococcus aureus Panton-Valentine Leukocidin (PVL) S subunit (LukS-PV)
This protein may be used in functional PVL toxicity assays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus.
Molecular Weight:Calculated: 33.713kD, including His tagObserved: ~30kD (SDS-PAGE)
Applications:Suitable for use in ELISA, Western Blot and Cytotoxicity assay. Other applications not tested.
Recommended Dilutions:ELISA: Internal QC showed detection down to 1ng/ml of LukS-PV protein when detected with monoclonal anti-LukS (Cat. #299605) as capture antibody and polyclonal anti-LukS (Cat. #299601) as detection antibody, followed by anti-rabbit IgG-HRP as secondary antibody.
Western Blot: Internal QC showed detection of 1ng of LukS-PV protein when detected with polyclonal anti-LukS antibody (Cat. #299601).
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukF-PV in a concentration range of 1-100nM.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
