This protein may be used in functional PVL toxicity assays in combination with LukS-PV, or as a control protein in ELISA assays or Western blotting when detecting LukF in PVL (+) strains of S. aureus.
Purified, Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV). The theoretical molecular weight of the protein is 35,580 Daltons.
Molecular Weight:~37kD
Applications:Suitable for use in ELISA, Western Blot and Cytotoxicity assay. Other applications not tested.
Recommended Dilutions:Western Blot: 100ngCytotoxicity assay: 1-100nMOptimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
