This protein may be used in functional toxicity assays in combination with rLukE, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
Purified, Staphylococcus aureus recombinant leukocidin-D (rLukD). The rLukD (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 36,889 Daltons.
Molecular Weight:~37kD
Applications:Suitable for use in ELISA, Western Blot and Cytotoxicity assay. Other applications not tested.
Recommended Dilutions:Western Blot: 100ngOptimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
