85-4425-28　［取扱停止］Zinc-alpha-2-Glycoprotein, Recombinant, Human (ZAG2) 10ug　299569

特徴

• Zinc-alpha-2-glycoprotein (ZAG) is found in body fluids such as serum, sweat, and seminal and breast cyst fluids. It is identical in amino acid sequence to tumor-derived lipid mobilizing factor (LMF), a protein associated with the dramatic loss of adipose body stores in cancer cachexia, and has been shown to stimulate lipolysis by adipocytes in vivo and in vitro. A role for ZAG has been proposed in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and also it regulates melanin production by normal and malignant melanocytes. It has also recently been classified as a novel adipokine in that it is produced by both white and brown fat adipocytes and may be useful for the study of Diabetes and obesity.
• Source:Recombinant protein corresponding to human ZAG2, a homodimeric, non-glycosylated polypeptide chain containing a total of 264aa, expressed in E. coli.
• Molecular Weight:~30.25kD
• Biological Activity:The ED50, as determined by the dose-dependant induction of IL-6 in primary human foreskin fibroblasts, was found to be 2ng/ml, corresponding to a specific activity of 5x10e5units/mg.
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ile-Thr-Ile-Pro.
• Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.