Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112aa polypeptide that remains associated with the latent portion of the molecule.
Source:Recombinant protein corresponding to human TGF Beta3, a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112aa, expressed in E. coli.
Molecular Weight:~25.45kD
Biological Activity:The ED50, as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison, is <0.05ng/ml, corresponding to a specific activity of 20MU/mg.
AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGFB3 as a Reference Standard.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 20% ethanol, 0.12% acetic acid.
