85-4425-05　［取扱停止］TIE-2, Fc Chimera, Recombinant, Human (Tyrosine Kinase with Ig and EGF Homology Domains-2, CD202b, TEK) 10ug　299543

特徴

• TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1122aa residue precursor protein with an 18 residue putative signal peptide, a 726 residue extracellular domain and a 353 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects.
• Source:Recombinant protein corresponding to human TIE-2, a monomeric, glycosylated, polypeptide containing 730aa, fused with the Fc part of human IgG1, expressed in Insect Cells.
• Molecular Weight:~250kD
• Biological Activity:Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4ug/ml (100ul/well), it binds rh Angiopoietin-2 with a linear range of 2-100ng/ml.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.