85-4424-83　［取扱停止］Platelet Derived Growth Factor AB, Recombinant, Human (PDGF-AB) 10ug　299510

特徴

• The term ‘PDGF’ refers to a family of disulphide bond-linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. The protein also contributes to wound healing and neural regeneration. There are four members of the PDGF family – PDGF A, PDGF B, PDGF C and PDGF D. Two distinct types of PDGF-A exist – a short form that is soluble and a long form that is retained by the extracellular matrix.
• Source:Recombinant protein corresponding to human PDGF-AB, a heterodimeric, non-glycosylated, polypeptide chain containing 234aa, expressed in E. coli.
• Molecular Weight:~25.5kD (13.3kD alpha-chain and 12.2kD beta-chain)Biological Activity:The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is <1ng/ml, corresponding to a specific activity of 1MIU/mg.
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Glu-Glu-alpha chain and Met-Ser-Leu-Gly-Ser-beta chain.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.