85-4424-53　［取扱停止］Matrix Metalloproteinase 7, Recombinant, Human (MMP7, Matrilysin, PUMP) 5ug　299450

特徴

• Matrix metalloproteinase-7 (MMP-7), also known as matrilysin and PUMP (EC 3.4.24.23), cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
• Source:Recombinant protein corresponding to human MMP7, a single, non-glycosylated, polypeptide chain, expressed in E. coli.
• Molecular Weight:~19.13kD
• Specific Activity:6,900U/mg
• Unit Definition:One unit is defined as the digestion of 1ug Azocoll/min at 37°C.
• Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
• Recommended Dilution:ELISA: 1ug/ml (to coat ELISA plates), detected with antibodies (1:10-50K neat serum, 0.5-1ug/ml affinity pure)Western Blot: 1:1K-5K (neat serum), 1-10ug/ml (affinity pure antibody using ECL technique) Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.