85-4424-52　［取扱停止］Matrix Metalloproteinase 3, Recombinant, Human, His-Tag (MMP3, Stromelysin-1, Transin-1) 10ug　299449

特徴

• MMP-3 enzyme is also known as Stromelysin-1 or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
• Source:Recombinant protein corresponding to a proform of the human MMP3 [Tyr18-Cys477 (Lys45Glu)], fused to His-tag at C-terminal, expressed in HEK293 cells.
• Molecular Weight:~52kD
• Biological Activity:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >150 pmoles/min/ug
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.