85-4424-39　［取扱停止］Leptin, Recombinant, Human, aa48-167, His-Tag (Ob, Obese Protein, Obesity Factor) 5ug　299438

特徴

• Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is ~16kD in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells.
• Source:Recombinant protein corresponding to human Leptin, a single, non-glycosylated, polypeptide chain containing aa48-167, fused to His-tag, expressed in E. coli.
• Molecular Weight:~19kD
• Applications:Suitable for use in Western Blot, Antibody Production and Protein Assays. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.