85-4424-18　［取扱停止］IL-17, Recombinant, Human (Interleukin-17, IL-17A, Interleukin-17A) 25ug　299422

特徴

• IL17 is a proinflammatory cytokine produced by activated T cells. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. Interleukin-17 can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.  It is also suspected to play a role in Autism.
• Source:Recombinant protein corresponding to human IL-17, a homodimeric, non-glycosylated polypeptide chain containing a total of 264aa, expressed in E. coli.
• Molecular Weight:~30.25kD
• Biological Activity:The ED50, as determined by the dose-dependant induction of IL-6 in primary human foreskin fibroblasts, was found to be 2ng/ml, corresponding to a specific activity of 5x10e5 Units/mg.
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ile-Thr-Ile-Pro.
• Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.