85-4424-03　［取扱停止］Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF) 2ug　299406

特徴

• GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes. 
• Source:Recombinant protein corresponding to human G-CSF, a single, non-glycosylated, polypeptide chain containing 175aa, expressed in E. coli.
• Molecular Weight:~18.8kD
• Biological Activity:The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), is <0.1ng/ml, corresponding to a specific activity of 1x10e8IU/mg.
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly.
• Protein Content: G-CSF quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.