85-4423-52　［取扱停止］Cyclin C, Recombinant, Human, His-Tag (CCNC) 10ug　299351

特徴

• rCCNC (cyclin C) is a member of the cyclin family of proteins. This protein interacts with cyclin-dependent kinase 8 (CDK8) and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this protein peaks in the G1 phase of the cell cycle. It has been shown that a non-cdk8-associated cellular pool of CCNC combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and that this phosphorylation is required for cells to exit GO efficiently. Other results have shown that a marked elevated immunoreactivity of CCNC was found both in neurons and astrocytes in Alzheimer's disease (AD). Increased levels of CCNC RNA were detected by ribonuclease protection assay (RPA) in severe AD cases and colocalization of CCNC and its preferred binding partner, CDK8, was only observed in astrocytes but not in neurons.
• Source:Recombinant protein corresponding to full length human Cyclin C, fused to a proprietary 16kD tag (a 6xHis tag located at the very N-terminal end), expressed in E. coli.
• Applications:Suitable for use directly as a positive control in Western Blot, ELISA, and Immunoprecipitation. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.