85-4423-45　［取扱停止］Ciliary Neurotrophic Factor, Recombinant, Human (CNTF) 5ug　299343

特徴

• CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor. CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
• Source:Recombinant protein corresponding to 199aa of human CNTF, expressed in E. coli.
• Molecular Weight:~22.71kD
• Biological Activity:The ED50 as determined by the dose-dependant stimulation of TF-1 cells is <2ng/ml.
• Specific Activity:5x10IU/mg
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.