85-4423-35　［取扱停止］CD14, Recombinant, Human (Monocyte Differentiation Antigen CD14, Myeloid Cell-Specific Leucine-Rich Glycoprotein) 50ug　299332

特徴

• CD14 is an ~50kD cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375aa residue precursor protein with a 19aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is ~65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP).
• Source:Recombinant protein corresponding to human CD14, expressed in CHO cells.
• Molecular Weight:~50kD
• Biological Activity:Up to 20ug/ml CD14 inhibits binding of LPS (FITC) (0.5ug/ml) to 6x10e5 CD14+CHO transfectants (FACS).
• Applications:Suitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.