85-4423-08　［取扱停止］Artemin, Recombinant, Human 5ug　299302

特徴

• The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of periferal neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described, two of which encode the same protein.
• Source:Recombinant protein corresponding to human Artemin, a disulfide-linked homodimer, non-glycosylated polypeptide chain containing 2x113aa, expressed in E. coli.
• Molecular Weight:~24.21kD
• Biological Activity:Calculated by it's ability to promote survival and neurite outgrowth and dorsal root ganglion neurons.
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gly-Gly-Pro-Gly.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.