Total RNA is isolated by modified guanidine thiocyanate techniques and stored in RNA storagebuffer.
Donor Information:Human, male 20,24,27,49 years old, pool of 4 donors, normal, brain: parietal lobeHuman, female 61 years old, pool of 1 donor, normal, brain: parietal lobe
Quality Control:1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8-2.1 (detected in 10mM Tris-HCl, pH 7.5)
2. The RNA is treated by DNAse I, and is tested as DNA free RNA by PCR
3. cDNA synthesis is successfully performed by using this RNA as template.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
