Tissue total protein is prepared from whole tissue homogenates.
Donor Information:Human, adult, total protein, Alzheimer's disease, brain: medulla oblongata
Quality Control:1. Protein lysate is verified on SDS-PAGE analysis as a consistent pattern
2. Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein
Applications:Suitable for use in Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction and tissue specific expression. Other applications not tested.
Recommended Dilution:Western Blot: 10-20ug/laneOptimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
