Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.
Sample Type:Cell or tissue extracts
Intended Use:Direct Assays: NADP+/NADPH concentrations and ratios in cell or tissue extracts.
Sensitivity:Detection limit 0.1um, linearity up to 10um
Specificity:This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH.
Test Principle:This NADP+/NADPH BioAssay™ kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is a convenient method to measure NADP, NADPH and their ratio.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
