Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.
Sample Type:Cell or tissue extracts
Intended Use:Direct Assays: NAD+/NADH concentrations and ratios in cell or tissue extracts.
Sensitivity:Detection limit of 0.05um and linearity up to 10um
Specificity:This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH.
Test Principle:This NAD+/NADH BioAssay™ kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565nm, is proportional to the NAD+/NADH concentration in the sample
This assay is a convenient method to measure NAD, NADH and their ratio.
Kit Components:Assay Buffer, 1x10ml Enzyme A, 1x120ulLactate, 1x1.5ml Enzyme B, 1x120ulMTT Solution, 1x1.5ml NAD Standard, 1x0.5mlNAD/NADH Extraction Buffers, 12ml each
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
