85-4422-28　［取扱停止］Luciferase Reporter Gene BioAssay™ Kit 500Tests　299021

特徴

• Intended Use:Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules
• Drug Discovery: high-throughput screen for gene modulators.
• Sensitivity:Detection of as little of 2fg luciferase and as few as 4 cells.
• Sample Type:Mammalian, yeast or E. coli cells
• Test Principle:The Luciferase Reporter Gene BioAssay™ is based on the quantitation of luciferase expression in mammalian, yeast or E. coli cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer
• Reaction scheme:LuciferaseATP+D-luciferin+O2 oxyluciferin+AMP+PPi+CO2+light
• This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kit includegene regulation studies and high-throughput screening of gene modulators.
• Data Analysis:The light intensity (RLU) is directly proportional to the luciferase concentration. For dose-response studies, the data are plotted against compound concentration and the EC50 for gene up-regulator compound and IC50 for a gene down regulator compound can be determined by non-linear regression analysis using Prism or other data analysis tools.
• Kit Components:Reagent, solidAssay Buffer, 1x20ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.