85-4422-26　［取扱停止］Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay™ Kit, Colorimetric 100Tests　299020

特徴

• Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higherconcentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors.
• Sample Type:Serum samples from any species
• Intended Use:Direct Assays: HDL and LDL/VLDL cholesterol in serum samples from any species
• Pharmacology: evaluation of drugs on cholesterol metabolism.
• Sensitivity:Detection limit of 5mg/dL, linearity up to 300mg/dL
• Test Principle:This HDL and LDL/VLDL Quantification BioAssay™ kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340nm is directly proportionate to the cholesterol concentration in the sample.
• Kit Components:PBS, 1x1.5ml Precipitation Reagent, 1x1.5mlAssay Buffer, 1x20ml Enzyme Mix, 1x120ulNAD Solution, 1x2ml Standard, 300mg/dL cholesterol, 1x1ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.