Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol fromcells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors.
Sample Type:Serum
Intended Use:Direct Assays: HDL and LDL/VLDL cholesterol in serum samples
Pharmacology: evaluation of drugs on cholesterol metabolism.
Range:1-100mg/dL cholesterol for colorimetric assays and 0.2-10mg/dL for fluorimetric assays
Test Principle:This HDL and LDL/VLDL quantification BioAssay™ kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm orfluorescence intensity at lem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
