Hemoglobin (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration.
Intended Use:Direct Assays: total hemoglobin in blood, serum, plasma, urine, etc. Pharmacology: effects of drugs on hemoglobin metabolism. Drug Discovery: HTS for drugs that modulate hemoglobin levels.
Range:0.9-200mg/dL
Test Principle:This Hemoglobin BioAssay™ Kit is based on an improved cyanohemiglobin method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and substantially reduces interference by substances in the raw samples.
Kit Components:Reagent, 1x50ml Calibrator, 1x10ml (equivalent to 100mg/dL hemoglobin)
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
