Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogenperoxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase.
Test Principle:This Glutathione BioAssay™ Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5'-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412nm, is directly proportional to glutathione concentration in the sample.
Key Features:
Sensitive and accurate: Linear detectionrange 0.4- 100 mMin 96-well plate
Simple and convenient: The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density
Low interference: Amino acids and common buffers do not interfere.
Applications:Direct Assays: Reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts
Drug Discovery/Pharmacology: Effects of drugs on glutathione metabolism.
Kit Components:299015A: Reagent A, 1x30ml 299015B: Reagent B, 1x30ml299015C: Calibrator, 1x10ml (equivalent to 100um glutathione)
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
