Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry.
Sample Type:Serum, plasma, urine and saliva samples
Intended Use:Direct Assays: ethanol in serum, plasma, urine and saliva samples. Pharmacology: effects of drugs on alcohol metabolism.
Sensitivity:Detection limit 0.0008 vol % (140um or 8ppm), linearity up to 0.1% ethanol
Test Principle:This Ethanol BioAssay™ kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565nm, is proportionate to the ethanol concentration in the sample.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.