Saliva or septum samples, the isolation procedure was designed for purification from 200ul of sample but may be used to isolate DNA from up to 600ul of saliva sample. If increasing the sample volume from 200ul, adjust the amount of Lysis Binding Buffer C, Proteinase K Solution, and Isopropanol proportionately.
Intended Use:The Saliva DNA Isolation BioAssay™ Kit is designed for the purification of genomic DNA and circulating DNA from freshly collected saliva samples.
Range:50-200ul of sample
DNA Yield:0.25-4ug/200ul fresh saliva
Test Principle:Purification is performed with the use of Spin columns which bind the DNA. No phenol-chloroform and precipitation steps are involved in this procedure. Isolation of the DNA is performed by lysing the cells in a solution containing chaotropic salts and detergent. To assist inlysing the cells, proteinase K is also added to the mixture. Isopropanol is added to the samples, before applying to the spin columns. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.
Kit Components:Lysis Binding Buffer C, 1x30ml, RTWash Buffer 1, 1x14ml, RTWash Buffer 2, 1x7ml, RTTE Buffer, 1x10ml, RTSpin column set, 50 units, RTProteinase K Solution, 1x250ul, -20°C* Wash buffers 1 and 2 are provided as concentrates that require the addition of 100% ethanol to them before use.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
