Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage.
Sample Type:Serum, plasma, and other biological samples
Intended Use:Direct Assays: cholesterol in serum, plasma, and other biological samples
Pharmacology: effects of drugs on cholesterol metabolism.
Range:1-100mg/dL cholesterol for colorimetric assays and 0.2-10mg/dL for fluorimetric assays.
Test Principle:This Cholesterol BioAssay™ uses a single working reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
