Calcium is measured to monitor diseases of the bone or calcium regulation disorders. Increased calcium levels in serum are reported in hyperparathyroidism, metastatic bone lesions and hypervitaminosis, while decreased levels are observed in hypoparathyroidism, nephrosis, rickets,steatorrhea, nephritis and calcium-losing syndromes. Urinary calcium levels aid the clinician in understanding how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the medical evaluation of kidney stones.
Sample Type:Biological samples
Intended Use:Direct Assays: Ca2+ in serum, urine, saliva etc
Drug Discovery/Pharmacology: effects of drugs on calcium metabolism
Food and Beverages: calcium determination
Environment: calcium determination in water and soil.
Range:0.08-20mg/dL (20um-5mM)
Test Principle:This Calcium BioAssay™ kit is designed to measure calcium directly in biological samples without any pretreatment. A phenolsulphonephthalein dye in the kit forms a very stable blue colored complex specifically with free calcium. The intensity of the color, measured at 612nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipid, protein and bilirubin.
Kit Components:Reagent A, 1x50mlReagent B, 1x50mlCalcium standard, 20mg/dL, 1x1ml
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
